Lightcycler 480 instrument operators manual software version 1. The lightcycler 480 system is a proven highperformance, mediumto high throughput pcr platform that provides various methods for gene detection, gene. Executed on roche lightcycler 480 realtime pcr system for safety and biohazard guidelines, refer to the safety section in the power sybr green pcr master mix and rtpcr protocol pn 4367218. Comparison of singleplex vs 4plex performance of roche lightcycler multiplex rna virus and taqpath 1step multiplex master mix no rox on a roche lightcycler 480 ii realtime pcr detection system. For more information, please consult chapter d, section 4. In combination with the lightcycler 480 system and suitable pcr primers, this kit allows very sensitive detection and quantification of defined dna sequences. The lightcycler 480 system is a highperformance, medium to highthroughput pcr platform 96 or 384well plates that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. See your instrument users manual for detailed instructions on how to load and run the plate.
Roche lightcycler 480 realtime pcr run setup instructions. The system features the lightcycler 480 instrument, a versatile, platebased realtime pcr device that supports mono or multicolor applications. Manually adding an analysisspecific tab to the sample editor. Lightcycler 480 realtime pcr system rapid by nature. When used with the lightcycler 480 system, this kit is ideally suited for hotstart pcr applications. Read the msds and follow the handling instructions. Form 1 below includes areas for recording control results and results for two patients. A pentium desktop pc is supplied with the instrument. Benefit from cuttingedge thermal block and optical technology that leads to excellent welltowell homogeneity and maximized detection. For optimal results, select an amplicon of 500 bp or less. If the instruments type is not stated, lightcycler 480 instrument stands for lightcycler 480 instrument i and ii.
Lightcycler 480 lightcycler 480 instrument ii 2 21 3. Roche applied science lightcycler 480 realtime pcr system. The lightcycler 480 ii with led is suitable for detection systems with different probe types such as hybridization probes or hydrolysis 5nuclease taqman probes. Roche lightcycler 480 realtime pcr setup instructions for epitect methyl ii pcr arrays. To generate a colour compensation file, the software must be opened by a user account that includes access rights for administrator or expert user. Lightcycler 480 instrument operators manual, lightcycler 480 led lamp spare lamp. The platebased lightcycler 480 system is a highly adaptable and versatile. Warranty information on warranty conditions are specified in the sales contract. Storage and stability storage conditions product the kit is shipped on dry ice.
Feb 29, 2012 automated dna isolation using roches magna pure lc 2. Enter sample information for your experiment as needed. Thirtytwo taqman gene expression assays were run in both 4plex 5 replicates and in simplex 3 replicates. Taqman fast advanced master mix on the roche lightcycler. N do not touch the surface of the the lightcycler 480 multiwell plate when. Manual for abi prism 7500 fast, lightcycler, mxpro v1. First presented in 2005, the lightcycler 480 realtime pcr system extends lightcycler system accuracy, speed, and versatility to medium and highthroughput applications in gene expression and genotyping analysis. Lightcycler 480 ii the lightcycler 480 ii instrument is used for performing rapid, accurate polymerase chain reaction pcr in combination with realtime, online detection enabling quantification of a target nucleic acid, as well as postpcr analysis of the amplified nucleic acid by melting curve analysis. Brilliant iii ultrafast sybr green qpcr master mix quick. Search results for lightcycler 480 at sigmaaldrich.
Proven, high performance the lightcycler 480 system is a proven highperformance, mediumto highthroughput pcr platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. Turn on the computer linked to the instrument and log on to window xp. The lightcycler 480 instrument ii 96 to 384well throughput, versatile for experienced users the lightcycler 480 instrument is a highperformance, flexible throughput qpcr platform with interchangeable 96 or 384well blocks that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and. Enter username and password when the login dialog box appears and click the button to proceed with the login. Brilliant iii ultrafast sybr green qrtpcr master mix. Get better performance from your roche lightcycler 480 ii. Start the lightcycler 480 software by doubleclicking the icon. Exporting individual lightcycler 480 software objects and experiment raw data.
Roche lightcycler 480 realtime pcr instrument standard operating procedure. Taqman fast advanced master mix on the roche lightcycler 480. Contents vial bottle cap label function description content 1 yellow lightcycler 480 control kit standard 1, 102 copies5. Lightcycler 480 instrument ii from roche sonoran surplus. V license statements for the lightcycler 480 multiple plate analysis software parts of the software used for the lightcycler 480 system are licensed from idaho. The lightcycler 480 realtime pcr system can detect this dye, but it will not be used for fluorescence normalization in the lightcycler 480 system software. In twostep rtpcr, the reverse transcription of rna into cdna is separated from the other reaction steps and is performed outside the. Transfering pcrs to hrmassays on the lightcycler 480 system examples for brca1. Lightcycler 480 ii sarscov 2 winterplex colour calibration instructions for use issue. Enter the username and assigned password to log in to the lightcycler 480 software. The use of other reagents has not been tested but is expected to work in the same way.
Lightcycler 480 instrument ii refer to section 12 of the instructions for use ifu 0009 for guidance in evaluating test results for run validity, interpretation of patient test results for a single pcr and section , and for interpretation of results for patient samples. See your instruments user manual for detailed instructions on how to configure the run method. N the performance of the kit as described in this instruction manual is war. Use or copy the software, in whole or in part, except as expressly provided in this agreement.
The following sections describe the portions of this manual you will find most. Use the software on more than one workstation at a time. Product description myco r eal pneumocystis is a realtime pcr assay for detection of dna. Tm ltd lightcycler 480 ii covid19 2g colour calibration. The lightcycler 480 system is a compact and versatile benchtop realtime pcr platform that can hold multiwell plates for either 96 or 384 samples the lightcycler 480 system is capable of running pcrs over a very broad range of volumes, ranging from 5 to 20.
Chlamydia pneumoniae cp real time pcr kit user manual. Crossing point cp values cp is equivalent to ct are used to determine unknown sample concentrations from a standard curve. Lightcycler 480 ii is a realtime pcr cycler for mid to high sample throughput. Available realtime rtpcr cycler and kinetic pcr machines. Benefit from cuttingedge thermal block and optical technology that leads to excellent welltowell homogeneity and. Contact addressesmanufacturerdistributiondistribution in usaroche instrument center agforrenstrassech6343 rotkreuzswitzerlandroche diagnostics. Application lightcycler 480 sybr green i master is designed for research studies. Reproducibility and sensitivity of realtime pcr on the lightcycler 480 instrument. Roche lightcycler 480 realtime pcr run setup instructions for rt2 profiler pcr arrays. Brilliant iii ultrafast sybr green qrtpcr master mix quick. This test was developed and validated for the lightcycler 1.
Protocols lightcycler 480 instrument protocol the following procedure is optimized for use with the lightcycler 480 instrument. Lightcycler 480 instrument operators manual citius. Contact your roche representative for further information. The lightcycler 480 system is a proven highperformance, mediumto highthroughput pcr platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. The lightcycler 480 multiple plate analysis software is intended for life science research only, not for use in diagnostic procedures. The assay design center will be available through december 31st, 2020. Program the lightcycler 480 instrument before preparing the reaction mixes. Revisions to this document are provided by roche diagnostics when necessary. Roche lightcycler 480 ii real time pcr system for 96 well. Serial dilutions of a viral target serial dilutions of a viral target sequence seven steps, 10 6 to 10 0 copies20 l were amplified via pcr and detected with hybprobe probes. Lightcycler 480 system georgia genomics and bioinformatics. Roche lightcycler whether your interest is in gene expression profiling or in detecting genetic variations, there is a member of the lightcycler system family offering the analytical performance and throughput you need for your research. Roche lightcycler 480 operators manual department of cell.
Tm ltd lightcycler 480 ii sarscov2 winterplex colour. Lightcycler carouselbased instruments and lightcycler 480 instrument. Ra lightcycler 480 ii nus yong loo lin school of medicine. A serial of 10fold dilutions of porcine gdna 10 2 104 ng were tested using lightcycler 480. Lightcycler 480 instrument operators manual version 1.
Executed on roche lightcycler 480 realtime pcr system for safety and biohazard guidelines, refer to the safety section in the fast sybr green master mix protocol pn 4385372. Follow the procedure below to prepare one 20 l standard reaction. Lightcycler 480 lightcycler 480 instrument ii 2 19 3. Any unauthorized modification of the instrument entails the invalidity of the guarantee and service. Print bookmark share pdf 1602kb english format file size language download get adobe reader contact qiagen. Wear appropriate protective eyewear, clothing, and gloves.
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